Location
- MRC Harwell
Introduction to Genetically Altered Mice
Who is this for?
- Animal Technicians
- Early Career Researchers
- PhD Students
This 2-day course will cover Mendelian gene inheritance as well as aspects of more complex genetics intended to inform breeding strategies and calculations in establishing new genetically altered lines.
After the course, you will be able to:
- Understand Mendelian gene inheritance
- Predict the outcomes of genetic crosses
- Have a working knowledge of the genetic background of mouse strains
- Understand how to maintain the genetic integrity of inbred lines
- Plan breeding schemes
- Perform multiple allele inheritance calculations
- Understand how Genetically Altered lines can be established from modern transgenic technologies
Hourly Schedule
Day 1
- 10:00 - 11:30
- Introductions / Session 1
- Registration. Orientation. Coffee. Icebreaker and H&S. Macromolecules. History of DNA. DNA to protein.
- 11:30 - 12:15
- Session 2
- Protiens
- 12:15 - 13:00
- Lunch
- Lunch is provided
- 13:00 - 13:30
- Session 3
- DNA to Disease Mechanism
- 13:30 - 14:30
- Session 4
- Inbred mice - why mice and why inbred?
- 14:30 - 14:50
- Break
- Refreshments provided
- 14:50 - 16:00
- Session 5
- Genetics drift. Nomenclature. Inbred strain difference. Outbred strains.
Day 2
- 9:30 - 10:30
- Session 1
- Cells, chromosomes, DNA replication. Mitosis and meiosis
- 10:30 - 11:00
- Session 2
- Dominant, recessive and human disorders
- 11:00 - 12:15
- Session 3
- Making genetic modifications
- 12:15 - 13:00
- Lunch
- Lunch is provided
- 13:00 - 13:45
- Session 4
- Coat colour and single allele inheritance
- 13:45 - 14:45
- Session 5
- Backcrossing and cohort breeding
- 14:45 - 15:00
- Break
- Refreshments provided
- 15:00 - 16:00
- Session 6
- Colony management. End of course
