Methods Development
The MLC contributes to the development of new techniques and research methods in areas such as animal husbandry and welfare, phenotyping of mutant lines, and mouse pathology. We are also at the forefront of improvements to technologies such as CRISPR and the latest imaging techniques.
Excess of guide RNA reduces knockin efficiency and drastically increases on-target large deletions
Chenouard, V., Leray, I., Tesson, L., Remy, S., Allan, A., Archer, D., Caulder, A., Fortun, A., Bernardeau, K., Cherifi, Y., Teboul, L., David, L., Anegon, I.
Dual-Stream Spatiotemporal Networks with Feature Sharing for Monitoring Animals in the Home Cage
Nwokedi, E. I., Bains, R. S., Bidaut, L., Ye, X., Wells, S., Brown, J. M.
An Improved Method for Growing Primary Neurons on Electron Microscopy Grids Co-Cultured with Astrocytes
Kumar, I., Paudyal, A., Kádková, A., Stewart, M., Sørensen, J. B., Radecke, J.
A method for TAT-Cre recombinase-mediated floxed allele modification in ex vivo tissue slices
Cheong, S. S., Luis, T. C., Stewart, M., Hillier, R., Hind, M., Dean, C. H.
Sectioning and Counting of Motor Neurons in the L3 to L6 Region of the Adult Mouse Spinal Cord
Austin, A., Beresford, L., Price, G., Cunningham, T., Kalmar, B., Yon, M.