A comprehensive list of all courses running at Advance

Course Portfolio

Advance Training Courses

Below is a list of all of the courses we run that can be searched by topic to find the right training for you. Not all will have currently available dates, if they weren’t scheduled for the current programme or have already taken place, but you can and should register interest to be notified when your course of interest is scheduled for the future. Course programmes with lists of scheduled courses are launched twice a year, so your course of interest should become available soon and courses that receive more interest are likely to be run more frequently.

To view currently running courses, please visit the course calendar.

Course Portfolio

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Genetics

Genetically altered mice remain one of the most commonly used animals in biomedical research and disease. As technology and science advance so does the complexity of animal models for genetic research. Understanding the background of mouse genetics, including allele crosses and the development of breeding strategies, is vital to advance medical research through the use of in vivo models.

Our genetics courses provide complete training, from an introduction to DNA and disease, through to an understanding of more advanced mouse genetics. Each course is designed to develop the knowledge and skills required by researchers and technicians to maintain high levels of expertise in this rapidly changing field.

Our genetics training includes the following courses:
  • DNA to Disease: Genetics for Beginners (1 day course)
  • Introduction to Genetically Altered Mice (2 day course)
  • Advanced Mouse Genetics (2 day course)
  • Breeding and Using Conditional Transgenic Models (1 day course)
  • Creating and Validating Conditional Transgenic Models (1 day course)
  • Introduction to Editing the Mouse Genome (1 day course)

Practical Transgenic Technologies

Over the last decade, the use of nucleases and more recently the highly efficient CRISPR-Cas9 has allowed gene targeting to be carried out directly into embryos and the rapid production of genetically altered (GA) mice, an essential tool in biomedical research.

With a range of techniques available for introducing CRISPR-Cas9 into the developing embryo, in addition to the use of cryopreservation to securely archive scientifically relevant mouse models, our Advance courses offer researchers the opportunity to develop their knowledge of genome editing and the importance of mouse strain preservation and protection.

Our Advance transgenic technology courses, led by experts in the field, are designed to teach and develop the necessary skills to keep up with current techniques when producing and archiving GA mouse lines.

Our transgenic technology training includes the following courses:

  • Mouse Embryo and Spermatozoa Cryopreservation (4 day course)
  • Microinjection and Electroporation (4 day course)

Mouse Necropsy, Histology and Pathology

Research pathology plays an important role in identifying and analysing disease phenotypes.

Appropriate use of pathology techniques will allow researchers to identify spontaneous and induced pathological changes, evidence of disease progression and the effect of experimental interventions.

  • Advanced Mouse Genetics

    Advanced Mouse Genetics

    Overview

    This course explores more complex genetic topics, including multiple allele crosses, complex disease models and will inform breeding strategies and experimental design rather than give a complete guide to the molecular biology. Our team of experienced trainers will support you to examine how different modifications lead to different breeding strategies, the importance of genetic consistency and integrity, different modes of inheritance and troubleshooting issues of reproducibility.

    After the course, you will be able to:
    • Understand the foundations of mouse genetics and its use in biomedical research
    • Have a working knowledge of different genetic backgrounds of mouse strains
    • Understand how to maintain the genetic integrity of inbred lines
    • Predict the outcomes of genetic crosses involving multiple alleles
    • Perform multiple allele inheritance calculations
    • Understand the fundamentals of different methods that give rise to Genetically Altered mice strains
    • Understand how Genetically Altered lines can be established from modern transgenic technologies
    • Troubleshoot breeding and colony maintenance issues
    • Nomenclature and access to international mouse resources
    Who is this for
    • Colony Managers
    • Researchers
    • PhD students
  • Breeding and Using Conditional Transgenic Models

    Breeding and Using Conditional Transgenic Models

    Overview

    This course focuses on breeding and using conditional transgenic models, including establishing colonies for conditional transgenesis and maintaining and breeding Genetically Altered colonies.

    After this course, you will be able to:
    • Have a working knowledge of different genetic backgrounds of mouse strains
    • Understand how to establish colonies for conditional transgenesis
    • Understand the maintenance and breeding of Genetically Altered colonies
    • Plan breeding schemes with consideration of control strategies and cohort numbers
    Who is this for
    • Animal Facility Managers
    • PhD students
    • Researchers with previous knowledge of advanced mouse genetics
  • Creating and Validating Conditional Transgenic Models

    Creating and Validating Conditional Transgenic Models

    Overview

    Transgenic mice are an important resource to understand gene function, regulation and expression. This course is designed to introduce the principles behind conditional genetic modifications, experimental design and analysis.

    After the course you will be able to:
    • Understand the basic principles of conditional transgenesis
    • Identify the advantages and challenges of these systems
    • Plan for restricting the expression of a transgene
    • Analyse recombinase (Cre) expression
    Who is this for
    • PhD students
    • Researchers with previous knowledge of advanced mouse genetics embarking on conditional transgenic projects
  • DNA to Disease: Genetics for Beginners

    DNA to Disease: Genetics for Beginners

    Overview

    What is a gene? What is DNA? How do these make proteins? What goes wrong and causes disease in the human body? This course provides a comprehensive introduction into genetics, covering the genetic code and how this leads to protein formation, as well as how errors in DNA can lead to disease.

    After this course, you will be able to:
    • Understand the concept of DNA and how genetic traits are inherited from one generation to the next
    • Understand what genes are and how they code for different proteins
    • Understand the role of different proteins in making different cell types
    • Understand some of the processes by which genetic disease can occur
    Who is this for
    • Animal Technicians
    • Technical Staff
    • Those who are new to genetics
  • Introduction to Editing the Mouse Genome

    Introduction to Editing the Mouse Genome

    Overview

    Genome editing of mice is widely used but can yield unpredictable outcomes. This course is designed to introduce the principles of design of genome editing, efficient strategies for screening of founder animals and comprehensive validation of newly established lines.

    After this course, you will be able to:
    • Understand the principles of design of CRISPR/Cas9 genome editing
    • Be aware of different strategies for screening founder animals
    • Understand validation strategies for newly established lines
    Who is this for
    • Researchers
    • PhD students
  • Microinjection and Electroporation

    Microinjection and Electroporation

    Overview

    The Microinjection and Electroporation course provides one-to-one tailored and hands-on training in embryo harvesting, set up and operation of microinjection rigs, pronuclear and cytoplasmic microinjection and the process of electroporation to introduce CRISPR-Cas9 reagents to embryos.

    In addition, this course offers a demonstration of surgical embryo transfer and the opportunity to meet and discuss your specific requirements with experts in IVF technology, CRISPR-Cas9 design and mouse colony management.

    Following this course, you will have:
    • Experience of harvesting and sorting 1-cell and 2-cell embryos
    • An understanding of the use of cryopreserved and IVF embryos in gene delivery
    • Practical training in pronuclear and cytoplasmic injection of CRISPR-Cas9 reagent into the embryo (1-cell and 2-cell embryos)
    • Practical training in electroporation of embryos
    • An understanding of the principles of colony management, IVF and cryopreservation
    Who is this for
    • Researchers
    • Senior Animal Technicians
    • PhD Students.
  • Mouse Embryo and Sperm Cryopreservation

    Mouse Embryo and Sperm Cryopreservation

    Overview

    Cryopreservation allows long-term storage of embryos and sperm, providing a convenient way to preserve, protect and transport mouse strains used in biomedical research. Archiving scientifically relevant mouse lines also adheres to good animal welfare practices by removing the need for continued animal breeding and is integral to any comprehensive colony management programme.

    Running bi-annually since 2004, this course provides practical experience in murine embryo and spermatozoa freezing techniques routinely used at MRC Harwell and a simple, robust in vitro fertilisation procedure. The course will also cover the basic laboratory skills required when working in an assisted reproductive biology environment such as pipetting, weighing, microscope use and embryo handling.

    Following this course, you will have:
    • Practical experience of sperm harvesting and freezing/thawing
    • A clear understanding of how to set up an in vitro fertilisation session using both frozen and freshly harvested sperm
    • Practical experience of oocyte and embryo harvesting for in vitro fertilisation and embryo freezing, respectively
    • Practical experience of embryo cryopreservation/thawing using a simple vitrification method
    • An understanding of surgical embryo transfer, including some practical experience of pre-operative aseptic techniques and sub-cuticular suturing
    • Practical experience of non-surgical embryo transfer techniques
    • An understanding of the current methods used for handling and shipping samples and managing a cryo-archive, as well as an overview of blastocyst genotyping
    Who is this for
    • Researchers
    • Senior Animal Technicians
    • PhD Students
  • Mouse Necropsy

    Mouse Necropsy

    Overview

    This course provides practical training in how to perform a basic standard mouse necropsy and describe gross observations made during the process.

    Following the course, you will be able to:
    • Perform a basic standard mouse necropsy
    • Describe gross observations at necropsy
    • Gain an understanding of the rapid ‘emergency’ opening
    Who is this for
    • Researchers
    • Animal Technicians
    • PhD Students.
  • Practical Laboratory Skills

    Practical Laboratory Skills

    Overview

    Having the knowledge and confidence to work safely in the laboratory is an essential skill for all staff working in laboratories. This course will cover basic theoretical and practical laboratory skills including preparing COSHH risk assessments, good laboratory practice, use of fume hoods/biosafety cabinets, centrifuges and other laboratory equipment, pipetting skills, using balances and preparing reagents and solutions.

    Following the course, you will be able to:
    • Prepare and understand the purpose of Control of Substances Hazardous to Health (COSHH) risk assessment (RA) forms
    • Understand the importance of good laboratory practice, including choosing appropriate personal protective equipment (PPE) and maintaining accurate laboratory records
    • Correctly use fume hoods, biosafety cabinets, centrifuges and other general laboratory equipment
    • Choose the correct pipette for your task and perform common techniques
    • Set up and use balances correctly
    • Prepare reagents and solutions
    • Adjust the pH of solutions
    Who is this for
    • Research Staff
    • Technical Staff
    • Students new to the laboratory environment, or experienced personnel looking to refresh their skills
  • Practical Light Microscopy

    Practical Light Microscopy

    Overview

    Proper microscope use is important for any researcher; using a microscope correctly prevents eyestrain, saves time and avoids the presence of artefacts in the final image. Our course will cover the background and development of the microscope and aims to develop researchers’ practice in microscopy.

    Following the course, you will be able to:
    • Set up a brightfield microscope for Köhler illumination
    • Understand how a microscope forms an image
    • Know when to use fluorescence and confocal microscopes
    Who is this for
    • Research Staff
    • Technical Staff
    • Students new to the laboratory environment, or experienced personnel looking to refresh their skills