Location
- MRC Harwell
Please email for future course interest
To get notified when this course is running again, view our course portfolio page
Please email for future course interest
Microinjection and Electroporation
The Microinjection and Electroporation course provides tailored and hands-on training in embryo harvesting, set up and operation of microinjection rigs, pronuclear and cytoplasmic microinjection and the process of electroporation to introduce CRISPR-Cas9 reagents to embryos.
In addition, this course offers a demonstration of surgical embryo transfer and the opportunity to meet and discuss your specific requirements with experts in IVF technology, CRISPR-Cas9 design and mouse colony management.
Who is this for?
- Researchers
- Senior animal technicians
- PhD students
Following this course, you will have:
- Experience harvesting and sorting 1-cell and 2-cell embryos
- An understanding of the use of cryopreserved embryos and IVF embryos in gene delivery
- Practical training in pronuclear and cytoplasmic injection of CRISPR-Cas9 reagent into the embryo
- Practical training of embryo electroporation
- An understanding of the principles of colony management, IVF and cryopreservation
